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【화학정보학】 생물학 실험에 사용되는 형광물질 라이브러리

 

생물학 실험에 사용되는 형광물질 라이브러리(dye library)

 

추천글 : 【생물학】 37강. 생물학 실험, 【화학정보학】 물질 라이브러리 


 

○ 1,8-ANS(1-anilinonaphthalene-8-sulfonic acid)

○ 6,8-Difluoro-7-hydroxy-4-methylcoumarin 

○ 7-Amino-4-methylcoumarin 

○ 7-Hydroxy-4-methylcoumarin

○ Acridine Orange

Alexa Fluor : 항체 등에 사용 시 labeling reaction을 거쳐야 함

Alexa Fluor 350 

○ Alexa Fluor 405 

○ Alexa Fluor 430 

○ Alexa Fluor 488 

○ Alexa Fluor 514 

○ Alexa Fluor 532 

○ Alexa Fluor 546 

○ Alexa Fluor 555 

○ Alexa Fluor 568 

○ Alexa Fluor 585 

Alexa Fluor 594 

○ Alexa Fluor 633 

○ Alexa Fluor 635 

○ Alexa Fluor 647  

○ Alexa Fluor 660

○ Alexa Fluor 680

○ Alexa Fluor 700

○ Alexa Fluor 750

○ Alexa Fluor 790

○ AMCA conjugate

○ Amino Coumarin

○ Auramine O

○ BFP(blue fluorescent protein)

○ BODIPY : excitation 493 nm, emission 503 nm

○ Calcein

○ Cascade Blue

○ CFDA

○ CFP(cyan fluorescent protein)

○ Coumarin

○ Cy series

Cy2 : excitation 489 nm, emission 506 nm

○ Cy3 : excitation (512);550 nm, emission 570;(615) nm

○ Cy3B : excitation 558 nm, emission 572;(620) nm

○ Cy3.5 : excitation 581 nm, emission 594;(640) nm

○ Cy5 : excitation (625);650 nm, emission 670 nm

○ Cy5.5 : excitation 675 nm, emission 694 nm

○ Cy7 : excitation 743 nm, emission 767 nm

○ DAPI : 핵 염색. 파란색 형광. DNA 부홈 내 AT region에 결합. apoptosis marker로도 사용

Di series : lipophilic particle에 처리해 주는 것만으로도 dye가 붙도록 할 수 있음 

○ DiA : lipophilic dye

○ DiD : lipophilic dye

○ DiI : lipophilic dye

○ DiO : lipophilic dye

○ DiR : lipophilic dye

○ DTAF

○ DyLight series

DyLight 350 : excitation 353 nm, emission 432 nm

○ DyLight 405 : excitation 400 nm, emission 420 nm

○ DyLight 488 : excitation 493 nm, emission 518 nm

○ DyLight 550 : excitation 562 nm, emission 576 nm

○ DyLight 594 : excitation 593 nm, emission 618 nm

○ DyLight 633 : excitation 638 nm, emission 658 nm

○ DyLight 650 : excitation 654 nm, emission 673 nm

○ DyLight 680 : excitation 692 nm, emission 712 nm

○ DyLight 755 : excitation 754 nm, emission 776 nm

○ DyLight 800 : excitation 777 nm, emission 794 nm

○ evoglow series

○ evoglow-Bs1

○ evoglow-Bs2

○ evoglow-Pp1

○ FA(fluorescein o-acrylate) : exication 490 nm, emission 514 nm

○ FDA

○ FITC : amine과 결합

○ Fluorescein

○ GFP series  

eCFP(enhanced cyan fluorescent protein)

eGFP(enhanced green fluorescent protein)

GFP(green fluorescent protein) : 자외선이 비춰지면 녹색의 형광을 발하는 단백질

○ RFP

○ YFP

○ Hoechst 33342 : 핵 염색. 파란색 형광

○ Hoechst 33258

○ ICG

○ Indo-1, Ca2+ : excitation 346 nm, emission 404 nm

○ Indo-1, Ca saturated : excitation 331 nm, emission 404 nm

○ IR series

○ IR-780

○ IR-797

○ IR-806

○ Lysotracker series

LysoSensor Blue 

○ LysoTracker Blue : excitation 373 nm, emission 422 nm

○ LysoTracker Green : excitation 504 nm, emission 511 nm

○ Luciferase

○ MitoTracker Deep Red

MitoTracker Green

○ mNeonGreen : excitation 506 nm, emission 517 nm

○ NBAM(nile blue acylamide) : excitation 628 nm, emission 667 nm

○ Nile Red

○ Pacific Blue

○ Rhodamine B

○ Rose bengal

○ Sapphire

○ SITS : 아민기를 특이적으로 표지하는 형광 물질

Sulfo cy5.5 amine

○ SureLight series

○ SureLight 488

○ SureLight APC

○ SureLight BPE

○ SureLight P3

○ SureLight PE-594

○ SureLight PE-665

○ SureLight PE-695

○ SureLight PE-777

○ SureLight PerCP

○ SureLight PerCP Cyanine 5.5

○ SureLight RPC

○ SureLight RPE

○ Syto60 : DNA dye

SYTOX Blue-DNA : 염색체와 결합하는 초록색 형광물질을 이용하여 죽은 세포의 양을 측정

○ Texas Red

○ TRITC

ViaFluor 488 : 미세소관 염색. 초록색 형광

 

입력 : 2022.05.04 11:50