【영상기기】 영상기기 개요

영상기기 개요

 

추천글 : 【생물학】 37강. 생물학 실험 

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1. BLI [본문]

2. FMT [본문]

3. US [본문]

4. SPECT [본문]

5. PET [본문]

6. MRI [본문]

7. CT [본문]

8. TPEM [본문]

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1. BLI(bioluminescence image) : preclinical only [목차]

⑴ spatial resolution : 5-20 mm depending on depth of signal

⑵ acquisition time : seconds

⑶ detection limit : ~ 10³ cells

⑷ 투과 깊이 : um ~ mm

① UV (150-380 nm) : 투과 깊이 ＜ 0.1 mm

② violet ~ deep blue (390-470 nm) : 투과 깊이 ~ 0.3 mm

③ blue ~ green (475-545 nm) : 투과 깊이 ~ 0.3-0.5 mm

④ yellow ~ orange (545-600 nm) : 투과 깊이 ~ 0.5-1.0 mm

⑤ red (600-650 nm) : 투과 깊이 ~ 1.0-2.0 mm

⑥ deep red ~ NIR (650-950 nm) : 투과 깊이 = 2-3 mm

⑦ NIR (950-1200 nm) : 투과 깊이 = 1 mm

○ image-guided surgery 등에 사용 

⑸ labeling strategy : reporter gene

⑹ advantages : cheap, simple, high throughout

⑺ disadvantages : small animals only, low resolution, only 2D images

 

 

2. FMT(fluorescence tomography) : preclinical only [목차]

⑴ spatial resolution : 2-3 mm

⑵ acquisition time : seconds to minutes

⑶ detection limit : ~ 10⁶ cells

⑷ imaging sensitivity : 10^-10 - 10^-12 mol/L

⑸ labeling strategy : reporter gene, fluorescence dye

⑹ 장점 : 낮은 비용, 단순성

⑺ 단점 : 낮은 해상도, 형광의 낮은 투과력

 

 

3. US(ultrasound) : preclinical and clinical [목차]

⑴ spatial resolution : 150 μm; -2 mm depending on depth

⑵ acquisition time : seconds to minutes

⑶ detection limit : not well characterized

⑷ 투과 깊이 : mm ~ cm

⑸ labeling strategy : reporter gene, antibody with microbubble

⑹ 장점 : 낮은 비용, 단순성

⑺ 단점 : 제한된 3D 이미징, 낮은 SNR(signal-to-noise ratio)

 

 

4. SPECT(single-photon emission computed tomography) : preclinical and clinical [목차]

⑴ spatial resolution : 0.5-2 mm (microSPECT), 7-15 mm (clinical SPECT)

⑵ acquisition time : minutes

⑶ detection limit : ~ 10⁵ cells

⑷ imaging sensitivity : 10^-10 - 10^-11 mol/L

⑸ 투과 깊이 : 제한 없음

⑹ labeling strategy : reporter gene, incubation with radiotracer

⑺ 장점 : 3D 이미징

⑻ 단점 : anatomic reference를 요한다는 점, radiation hazard

 

 

5. PET(positron emission tomography) : preclinical and clinical [목차]

⑴ spatial resolution : 1-2 mm (microPET). 4-10 mm (clinical PET)

⑵ acquisition time : seconds to minutes

⑶ detection limit : ~ 10⁴ cells

⑷ imaging sensitivity : 10^-11 - 10^-12 mol/L

⑸ 투과 깊이 : 제한 없음

⑹ labeling strategy : reporter gene, incubation with radiotracer

⑺ 장점 : 3D 이미징, 조영제의 높은 sensitivity, 기능적 이미징

⑻ 단점 : 비용, anantomic reference를 요한다는 점, 낮은 공간적 해상도, radiation hazard

 

 

6. MRI(magnetic resonance imaging) : preclinical and clinical [목차]

⑴ spatial resolution : 0.01-0.1 mm (small animal MRI), 0.5-1.5 mm (1.5 T MRI)

⑵ acquisition time : minutes to hours

⑶ detection limit : ~ 10⁴ cells

⑷ imaging sensitivity : 10^-3 - 10^-5 mol/L

⑸ 투과 깊이 : 제한 없음

⑹ labeling strategy : internalization or surface labeling with nanoparticles or specific ions

⑺ 장점 : 3D 이미징, 높은 공간적 해상도, 방사능을 요하지 않는다는 점, soft tissue의 contrast가 좋다는 점

⑻ 단점 : 높은 비용, 복잡한 원리, 오래 걸린다는 점

 

 

7. CT(computed tomography) : preclinical and clinical [목차]

⑴ spatial resolution : 0.02-0.3 mm (microCT), 0.5-2 mm (clinical CT) 

⑵ acquisition time : seconds to minutes

⑶ detection limit : not well characterized

⑷ imaging sensitivity : 10^-2 - 10^-3 mol/L

⑸ 투과 깊이 : 제한 없음

⑹ labeling strategy : internalization or surface labeling with nanoparticles

⑺ 장점 : 3D 이미징, 상대적으로 낮은 가격, 높은 공간적 해상도, 빠른 스캔 시간

⑻ 단점 : radiation hazard, 조영제의 낮은 sensitivity

 

 

8. TPEM(two photon excitation microscopy) : preclinical only [목차]

 

입력: 2021.03.07 12:12