【생물학】 동물실험 5강. 종양모델

동물실험 5강. 종양모델

 

추천글 : 【생물학】 30강. 생물학 실험 

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1. spontaneous [본문]

2. virus-induced [본문]

3. transgenic [본문]

4. induced / carcinogens [본문]

5. transplantation [본문]

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출처 : Datta, Amrita, and Debasis Mondal. "Development of mouse models for cancer research." Animal Biotechnology. Academic Press, 2014. 73-94.

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1. spontaneous [목차]

⑴ 추후 업데이트

 

 

2. virus-induced [목차]

⑴ classic models of virus-induced leukemia

① Rauscher

② Moloney

③ LP-BMS

④ Friend

⑵ non-leukemic tumor

① mammary tumors due to MMTV

② thymomas of AKR mice

 

 

3. transgenic [목차]

⑴ GEM mice (knock-out/in) 

① lung adenocarcinoma : K

② breast ductal carcinoma : Brca2, Trp53

③ breast cancer : MMTV-PyMT

④ prostate carcinoma : Pten, Pten-Nkx.1, Rb1-Trp53

⑤ liver carcinoma : Apc, Myc-Trp53, Myc-TGFA

⑥ http://emice.nci.nih.gov/emice/mouse_models 

 

 

4. induced / carcinogens [목차]

⑴ carcinogens

① DMBA [initator mutagen] followed by TPA

② 1,2-dimethylhydrazine-2-HCl

③ zzoxymethane

④ nitrosamine-4-(methyl-nitrosamiine)-1(3-pyridyl)-1-butanone

⑤ methylcholanthrene

⑵ epithelial tumorigenesis

⑶ GI tumorigenesis

⑷ sarcoma induction

⑸ lung tumorigenesis

 

 

5. 이식(transplanted) [목차]

⑴ 정의 : 이식에 따른 육안 평가 또는 현미경 관찰

⑵ 종류 1. 자가이식(autograft)

① 정의 : 자기의 세포 또는 조직을 이식하는 것

⑶ 종류 2. 동형이식(syngeneic)

① 정의 : same species, same strain

② B16-F10 tumors in C57BI/6 mice. cold tumor. high growth rate

③ 4T1 : breast cancer cell line. cold tumor

④ CT26 : hot tumor

⑷ 종류 3. 이형이식(allograft, allogeneic)

① 정의 : same species, different strain

② M5076 sarcomas in athymic mice

⑸ 종류 4. 동계이식(isograft)

① 이형이식 중 쌍둥이와 같이 굉장히 가까운 사람으로부터 이식받는 것

⑹ 종류 5. 이종이식(xenogeneic)

① 정의 : different species

② U87-MG

③ human tumors growth in immunocompromised mice

④ patient-derived xenograft tumor model : PDX (breast cancer), etc.

⑺ implant site

① orthotopic : 특정 세포를 그 세포가 유래한 조직에 이식하는 방식으로 비교적 어려운 편

② heterotopic (ectopic) : orthotopic이 아닌 방식

 

입력: 2021.10.06 22:10