【연구】 A high stable pH-temperature dual-sensitive liposome for tuning anticancer drug release

Zhao, Y., Cai, F., Shen, X., & Su, H. (2020). A high stable pH-temperature dual-sensitive liposome for tuning anticancer drug release. Synthetic and Systems Biotechnology, 5(2), 103-110.

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1. Terminology

⑴ DPPC : 1,2-dipalmitoyl-sn-glycero-3-phosphocholine 

⑵ DC-Chol : 3β[N-(N',N'-dimethyl aminoethane)-carbamoyl]cholesterol

⑶ CPTSL : composite pH-temperature dual-sensitive liposome

⑷ PTSL : pH-temperature dual-sensitive liposoem

⑸ PASP-g-C8 : octylamine-graft-poly aspartic acid 

2. CPTSL의 제법

⑴ 30 mg DPPC, 6 mg DC-Chol, 1.5 mg cholesterol을 3.5 mL CH3Cl에 녹임

① DPPC : Corden Pharma Switzerland LLC (Switzerland)에서 구매

② DC-Chol : Absin Bioscience Inc. (China)에서 구매 

③ cholesterol : Beijing Aoboxing Biotechnology Co. Ltd. (China)에서 구매

⑵ model drug으로 정한 CTY 12 mg/mL를 1 mL 추가해 줌

⑶ mixture에 2분 동안 ultrasound를 처리해 주어 W/O emulsion을 형성시킴

⑷ 12 mL PASP-g-C8 수용액을 첨가

① 참고 : Wang, L., Geng, D., & Su, H. (2014). Safe and efficient pH sensitive tumor targeting modified liposomes with minimal cytotoxicity. Colloids and Surfaces B: Biointerfaces, 123, 395-402.

 

Figure. 1. The structure and mechanism of PLPs

a. the preparation of PASP

b. the structure of PLPs

c. the operating mode of PLPs in a pH 5.0 solution

d. the operating mode of PLPs in a pH 7.4 solution 

 

② PSI : Luoyang Mining Run Environmental Protection Material Co. Ltd. (China)

③ PASP : Kang'en Chemical Co. Ltd. (China)

④ Octylamine : Beijing Kehua Jingwei Tech. Co. Ltd. (China)

⑸ 30 ℃, 1 h 하에서 CH3Cl을 vaccum rotary evaporation을 통해 증발시켜 W/O/W emulsion을 형성시킴

3. PTSL의 제법 

⑴ 30 mg DPPC, 7 mg DC-Chol을 3.5 mL CH3Cl에 녹임

⑵ 이하의 과정은 CPTSL과 동일함